This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Includes a glossary of over 200 terms important for understanding molecular biology Uses an inexpensive source of eukaryotic cells - great for schools on a budget Includes Methods Locator that provides instant access to the latest methods Contain clearly written, easy-to-follow, student-tested instructions: Sterile techniques Phage titration Gel electrophoresis of DNA Restriction enzyme digestion Plasmid isolation Transformation of E. Coli Recombinant DNA cloning Nick translation labeling Nonradioactive primer labelling Nonradioactive DNA detection Southern blotting Colony hybridization Purification of plant DNA RNA purification Northern blotting Purification of poly A+ RNA Polymerase chain reaction (PCR)
FIGURE 6.20 Real-Time PCR With SYBR Green When the fluorescent dye SYBR Green I is present during a PCR reaction, it binds to the doublestranded PCR product and emits light at 520 nm. The SYBR Green dye only fluoresces when bound to DNA ...
The Problems Book helps students appreciate the ways in which experiments and simple calculations can lead to an understanding of how cells work by introducing the experimental foundation of cell and molecular biology.
Focuses on the fundamental aspects of molecular structure and funciton by reviewing key features, and along the way, capsulizing them as a series of concise concepts.
Molecular Biology and Biotechnology
The majority of discussions have been modified to reflect the latest changes in the field. The book also builds on its strong illustration program by opening each chapter with “VIP” art that serves as a visual summary for the chapter.
This book is an essential guide for young scientists on their way to research success in this exciting field.
This laboratory manual gives a thorough introduction to basic techniques.
This volume contains the papers presented at RECOMB 2009: the 13th Annual International Conference on Research in Computational Molecular Biology held in Tucson, Arizona, USA, during May 18-21, 2009.
PART I Molecular Biology 1. Molecular Biology and Genetic Engineering Definition, History and Scope 2. Chemistry of the Cell: 1. Micromolecules (Sugars, Fatty Acids, Amino Acids, Nucleotides and Lipids) Sugars (Carbohydrates) 3.
The text has been divided into two parts. Part I includes thirteen chapters that deal with the general principles of the molecular biology of cancer that provide the basic framework for an understanding of the behavior of cancer cells.