Features, Provides a comprehensive treatment of fluorescence lifetime spectroscopy and imaging using both endogenous and exogenous probes, Includes a review of tissue autofluorescence and its molecular origins, Discusses the fundamentals, state-of-the-art techniques, and their current applications in tissue diagnostics for research and clinical applications, Offers a survey of instrumentation and analysis of fluorescence lifetime data, including global analysis and computational approaches, Covers the wide range of clinical applications in oncology, cardiovascular, metabolism, dermatology, tissue engineering, and ophthalmology, as well as photodynamic therapy and tomographic FLIM of animals, Edited by pioneers in this field, with contributions from Leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery. Book jacket.