Immunohistochemistry and Immunocytochemistry: Essential Methods

Immunohistochemistry and Immunocytochemistry: Essential Methods
ISBN-10
1118717740
ISBN-13
9781118717745
Category
Science
Pages
248
Language
English
Published
2017-02-15
Publisher
John Wiley & Sons
Author
Simon Renshaw

Description

Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.

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