The use of the chemical modification of proteins has evolved over the past 80 years, benefiting from advances in analytical, physical, and organic chemistry. Over the past 30 years, the use of chemical reagents to modify proteins has been crucial in determining the function and structure of purified proteins. This groundbreaking work is part of the foundation of emerging disciplines of proteomics, chemical biology, structure biology, and chemical proteomics. Chemical Reagents for Protein Modification, Fourth Edition provides a comprehensive review of reagents used for the chemical modification of proteins, representing a major revision of the work presented in previous editions. The completely updated Fourth Edition is substantially larger and includes five new chapters: Alkylating Agents Acylating Agents Nitration and Nitrosylation Oxidation Modification of Proteins with Reducing Agents There is greatly increased coverage of the chemical modification of cysteine, which is critical for bioconjugate synthesis. The chapter on reduction also provides information necessary for bioconjugate synthesis as well as for the processing of inclusion bodies. The book places emphasis on conditions that affect the specificity of the chemical modification of proteins, such as solvent and temperature. The format has been markedly revised, presenting information based on the chemical nature of the modifying material and on the amino acid residue modified. This new version has increased significance to biopharmaceuticals. Much of the information is in tabular form, which enables the rapid location of cited material.
This groundbreaking work is part of the foundation of emerging disciplines of proteomics, chemical biology, structure biology, and chemical proteomics.
The completely updated text is substantially larger and includes five new chapters: Alkylating Agents, Acylating Agents, Nitration and Nitrosylation, Oxidation, and Modification of Proteins with Reducing Agents. The
Edited by renowned protein scientist and bestselling author Roger L. Lundblad, with the assistance of Fiona M. Macdonald of CRC Press, this fourth edition of the Handbook of Biochemistry and Molecular Biology represents a dramatic revision ...
... Ed. Bioconjugate Techniques, 3rd Edition, Thermo Fisher Scientific, Rockford, IL, USA, Published: August 2013, Imprint: Academic Press, ISBN: 978-0-12-382239-0 Lundblad, R.L. Chemical Reagents for Protein Modification, 4th Edition, ...
Particular emphasis is placed on the use of photoaffinity reagents. The book will be an extremely useful research tool for all investigators interested in the solution chemistry of proteins.
C.F. Poole, Liquid chromatography: Chapter 2, Derivatization in Liquid Chromatography, Kindle Edition, Elsevier, Oxford, UK, 2013. 22. R.L. Lundblad, Chemical Reagents for Protein Modification, 4th ed., CRC Press, Taylor & Francis Group ...
Anal. Bioanal. Chem. 397, 2401–2407. Olekhnovich, I.N., Kadner, R.J., 2002. Mutational scanning and affin- ity cleavage analysis of UhpA-binding sites in the Escherichia coli uhpT promoter. J. Bacteriol. 184, 2682–2691. Osborn ...
The Robinson annulation sequence is a powerful method for the construction of sixmembered rings . It is therefore no surprise that this sequence has been widely applied in steroid synthesis . Beginning with the bicyclic ketone shown in ...
The modification of each of these cysteines by the impermeant fluorescent reagent fluorescein 5-maleimide ... in the much larger segments connecting the second and the third candidate and following the fourth candidate for spanning the ...
Peptide map analysis is a technique that is routinely applied during various protein product characterization studies. ... The amino acids on the parent mAb are likely to undergo chemical modifications (e.g., isomerization, deamidation, ...