Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
ISBN-10
1475753489
ISBN-13
9781475753486
Category
Science
Pages
632
Language
English
Published
2013-04-17
Publisher
Springer Science & Business Media
Author
James Pawley

Description

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

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