Peptide Synthesis and Applications
- ISBN-10
- 1592598773
- ISBN-13
- 9781592598779
- Category
- Science
- Pages
- 263
- Language
- English
- Published
- 2008-02-02
- Publisher
- Springer Science & Business Media
- Author
- John Howl
Description
Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.
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