Multi-Parametric Live Cell Microscopy of 3D Tissue Models
- ISBN-10
- 3319673580
- ISBN-13
- 9783319673585
- Category
- Science
- Pages
- 172
- Language
- English
- Published
- 2017-10-26
- Publisher
- Springer
- Author
- Ruslan I. Dmitriev
Description
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
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