CELL AND MOLECULAR BIOLOGY: A Lab Manual

CELL AND MOLECULAR BIOLOGY: A Lab Manual
ISBN-10
8120348001
ISBN-13
9788120348004
Series
Cell and Molecular Biology
Category
Science
Pages
156
Language
English
Published
2013-06-21
Publisher
PHI Learning Pvt. Ltd.
Author
K. V. Chaitanya

Description

This laboratory guide, intended for undergraduate and postgraduate students, includes techniques and their protocols ranging from microscopy to in vitro protein synthesis. Experiments relating to chromosomes study and identifying the phases of cell division are explained. The book lucidly deals with the extraction and characteri-zation of chromatin and techniques for studying its modifications, the gene methodology for identification of mutation and the methodology for isolation of nucleic acids from all types of organisms, such as viruses, fungi, plants and animals. All the protocols have been explained following step-by-step method. Different types of electrophoresis and their techniques, including blotting techniques and the methodology for stripping of probes from membranes for reusing the blot, have also been dealt with. Protocols on modern molecular biology techniques—PCR, restriction enzyme digest, DNA isolation, cloning and DNA sequencing—add weightage to the book. It also gives necessary knowledge of different types of stains, staining techniques, buffers, reagents and media used in the protocols. To help students prepare for answering viva voce questions, the book includes MCQs based on the discussed techniques.

Get the book

Amazon.com
Amazon.com
Search the book
eBay.com
eBay.com
Search the book

Other editions

Similar books