PART I Molecular Biology 1. Molecular Biology and Genetic Engineering Definition, History and Scope 2. Chemistry of the Cell: 1. Micromolecules (Sugars, Fatty Acids, Amino Acids, Nucleotides and Lipids) Sugars (Carbohydrates) 3. Chemistry of the Cell . 2. Macromolecules (Nucleic Acids; Proteins and Polysaccharides) Covalent and Weak Non-covalent Bonds 4. Chemistry of the Gene: Synthesis, Modification and Repair of DNA DNA Replication: General Features 5. Organisation of Genetic Material 1. Packaging of DNA as Nucleosomes in Eukaryotes Techniques Leading to Nucleosome Discovery 6. Organization of Genetic Material 2. Repetitive and Unique DNA Sequences 7. Organization of Genetic Material: 3. Split Genes, Overlapping Genes, Pseudogenes and Cryptic Genes Split Genes or .Interrupted Genes 8. Multigene Families in Eukaryotes 9. Organization of Mitochondrial and Chloroplast Genomes 10. The Genetic Code 11. Protein Synthesis Apparatus Ribosome, Transfer RNA and Aminoacyl-tRNA Synthetases Ribosome 12. Expression of Gene . Protein Synthesis 1. Transcription in Prokaryotes and Eukaryotes 13. Expression of Gene: Protein Synthesis: 2. RNA Processing (RNA Splicing, RNA Editing and Ribozymes) Polyadenylation of mRNA in Prokaryotes Addition of Cap (m7G) and Tail (Poly A) for mRNA in Eukaryotes 14. Expression of Gene: Protein Synthesis: 3. Synthesis and Transport of Proteins (Prokaryotes and Eukaryotes) Formation of Aminoacyl tRNA 15. Regulation of Gene Expression: 1. Operon Circuits in Bacteria and Other Prokaryotes 16. Regulation of Gene Expression . 2. Circuits for Lytic Cycle and Lysogeny in Bacteriophages 17. Regulation of Gene Expression 3. A Variety of Mechanisms in Eukaryotes (Including Cell Receptors and Cell Signalling) PART II Genetic Engineering 18. Recombinant DNA and Gene Cloning 1. Cloning and Expression Vectors 19. Recombinant DNA and Gene Cloning 2. Chimeric DNA, Molecular Probes and Gene Libraries 20. Polymerase Chain Reaction (PCR) and Gene Amplification 21. Isolation, Sequencing and Synthesis of Genes 22. Proteins: Separation, Purification and Identification 23. Immunotechnology 1. B-Cells, Antibodies, Interferons and Vaccines 24. Immunotechnology 2. T-Cell Receptors and MHC Restriction 25. Immunotechnology 3. Hybridoma and Monoclonal Antibodies (mAbs) Hybridoma Technology and the Production of Monoclonal Antibodies 26. Transfection Methods and Transgenic Animals 27. Animal and Human Genomics: Molecular Maps and Genome Sequences Molecular Markers 28. Biotechnology in Medicine: l.Vaccines, Diagnostics and Forensics Animal and Human Health Care 29. Biotechnology in Medicine 2. Gene Therapy Human Diseases Targeted for Gene Therapy Vectors and Other Delivery Systems for Gene Therapy 30. Biotechnology in Medicine: 3. Pharmacogenetics / Pharmacogenomics and Personalized Medicine Phannacogenetics and Personalized 31. Plant Cell and Tissue Culture' Production and Uses of Haploids 32. Gene Transfer Methods in Plants 33. Transgenic Plants . Genetically Modified (GM) Crops and Floricultural Plants 34. Plant Genomics: 35. Genetically Engineered Microbes (GEMs) and Microbial Genomics References
The availability of genome sequences means that computer-based searches can be used to find particular genes. ... As we saw when looking at primer design for PCR (Section 7.2.2), a mixed probe can be synthesised that covers all the ...
An authoritative yet easy-to-read description of molecular biology, genetics, and the ethical implications of genetic engineering.
This book offers a clear and engaging exploration of the principles of molecular biology and genetic engineering, providing readers with a profound understanding of the underlying mechanisms shaping life.
Authored by an integrated committee of plant and animal scientists, this review of newer molecular genetic techniques and traditional research methods is presented as a compilation of high-reward opportunities for agricultural research.
Although designed for undergraduates with an interest in molecular biology, biotechnology, and bioengineering, this book-Techniques in Genetic Engineering-IS NOT: a laboratory manual; nor is it a textbook on molecular biology or ...
... 142 Boswell , James G. , 171 Boveri , Theodore , 16 Boyer , Herbert , 187 , 189 , 201 Boyer's group , 187 Brachet , Jean , 126 , 140 , 146 Bragg , Lawrence , 105 , 106 , 109 , 112 Bragg , William , 112 Bragg's group , 105 , 106 ...
Comprised of chapters authored by leading experts in their respective fields, this authoritative reference text:Highlights the latest omics-ba
The author presents a basic introduction to the world of genetic engineering. Copyright © Libri GmbH. All rights reserved.
The book discusses baker's yeast, in addition to a number of unconventional yeasts being used in an increasing number of studies. 175 figures help illustrate the information presented.
Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists.This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and ...